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rho activation assay kit  (Cell Biolabs Inc)

 
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    Cell Biolabs Inc rho activation assay kit
    Rho Activation Assay Kit, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rho+activation+assay+kit/activation+assay+cdc42+combo+kit+rac1+rhoa/pm41857010-157-9-13
    Average 86 stars, based on 1 article reviews
    rho activation assay kit - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    Activation Assay:

    Article Title: Histone lactylation-driven feedback loop modulates pyrimidine metabolism to promote oral carcinogenesis.
    Article Snippet: A Vanquish UHPLC coupled to a TSQ Altis triple quadrupole mass spectrometer (Thermo Fisher, Massachusetts, USA) was utilized to analyze the clear metabolite extract. .. The levels of GTP-bound RhoA were measured using a Rho Activation Assay Kit (Cell Biolabs, CA, USA) in accordance with the manufacturer’s guidelines. ..

    Article Title: Regulation of bone homeostasis by MERTK and TYRO3.
    Article Snippet: .. GTP-bound RHOA was analyzed by performing a pull-down assay with Cell Biolabs, Inc. Rho Activation Assay kit. ..

    Article Title: Leptin stimulates migration and invasion and maintains cancer stem-like properties in ovarian cancer cells: an explanation for poor outcomes in obese women
    Article Snippet: .. RhoA pull-down assays were performed using the Rho activation assay kit according to the manufacturer's instructions (Cell Biolabs Inc., San Diego, CA, USA). ..

    Article Title: Leptin stimulates migration and invasion and maintains cancer stem-like properties in ovarian cancer cells: an explanation for poor outcomes in obese women.
    Article Snippet: .. RhoA pull-down assays were performed using the Rho activation assay kit according to the manufacturer’s instructions (Cell Biolabs Inc., San Diego, CA, USA). ..

    Article Title: TAM receptors control actomyosin dynamics in osteoclasts via RHOA-COFILIN-MYOSIN II signaling
    Article Snippet: .. GTP-bound RHOA was analyzed by performing a pull-down assay with Cell Biolabs, Inc. Rho Activation Assay kit. ..

    Article Title: Histone lactylation-driven feedback loop modulates pyrimidine metabolism to promote oral carcinogenesis
    Article Snippet: A Vanquish UHPLC coupled to a TSQ Altis triple quadrupole mass spectrometer (Thermo Fisher, Massachusetts, USA) was utilized to analyze the clear metabolite extract. .. The levels of GTP-bound RhoA were measured using a Rho Activation Assay Kit (Cell Biolabs, CA, USA) in accordance with the manufacturer’s guidelines. ..


    Pull Down Assay:

    Article Title: Regulation of bone homeostasis by MERTK and TYRO3.
    Article Snippet: .. GTP-bound RHOA was analyzed by performing a pull-down assay with Cell Biolabs, Inc. Rho Activation Assay kit. ..

    Article Title: TAM receptors control actomyosin dynamics in osteoclasts via RHOA-COFILIN-MYOSIN II signaling
    Article Snippet: .. GTP-bound RHOA was analyzed by performing a pull-down assay with Cell Biolabs, Inc. Rho Activation Assay kit. ..

    other:

    Article Title: Simvastatin interferes with cancer ‘stem-cell’ plasticity reducing metastasis in ovarian cancer
    Article Snippet: Peroxidase-conjugated goat anti-202 mouse/rabbit/goat IgGs were used as secondary antibodies (Bio-Rad).



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    <t>RHOA</t> and MYO9B play roles in the ability of the RGDKGE collagen peptide (P2) to modulate CD8 + T-cell migration on denatured collagen-IV (Den-Coll-IV). A and B: Whole cell lysates were prepared from Jurkat T cells treated with the P2 or control peptide (CP; A ) or dimethyl sulfoxide (DMSO) buffer control or RHOA inhibitor rhosin ( B ) and analyzed by Western blot analysis for total and activated RHOA using an active <t>RHO</t> detection kit. C and D: Quantification of Jurkat T-cell migration on denatured collagen-IV ( C ) or native collagen-IV (Nat-Coll-IV; D ) in the presence of DMSO or RHOA inhibitor rhosin. C: Data represent change in cell migration from four independent experiments with control DMSO treatment set to 100% for comparison. D: Data represent change in cell migration from three independent experiments with control DMSO treatment set to 100% for comparison. E and F: Cell lysates were prepared from Jurkat T cells treated with the P2 or CP ( E ) or DMSO or SRC non-receptor tyrosine kinase (SRC) inhibitor ( F ), and analyzed by Western blot analysis for MYO9B. G: Western blot analysis for MYO9B levels in control or MYO9B-specific siRNA-transfected Jurkat T cells. H and I: Quantification of control and MYO9B-specific knockdown Jurkat T-cell migration on denatured collagen-IV ( H ) or native collagen-IV ( I ). H: Data represent change in cell migration from four independent experiments with control siRNA treatment set to 100% for comparison. I: Data represent change in cell migration from two independent experiments with control siRNA treatment set to 100% for comparison. J: Quantification of MYO9B knockdown Jurkat T-cell migration on denatured collagen-IV in the presence of CP or P2. Data represent change in cell migration from three independent experiments with control CP treatment set to 100% for comparison. K: Example of F-actin polarization ( arrows ) in control or MYO9B-specific siRNA-transfected Jurkat T cells seeded onto denatured collagen-IV. L: Quantification of the mean percentage of F-actin polarized cells per ×400 field in control and MYO9B knockdown Jurkat T cells seeded onto denatured collagen-IV. Data represent percentage of F-actin polarized T cells from two independent experiments calculated from 10 ×400 microscopic fields per condition. Data are given as means ± SEM ( C , D , H – J , and L ). ∗ P < 0.05, ∗∗ P < 0.01. Scale bars = 20 μm ( K ).
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    RHOA and MYO9B play roles in the ability of the RGDKGE collagen peptide (P2) to modulate CD8 + T-cell migration on denatured collagen-IV (Den-Coll-IV). A and B: Whole cell lysates were prepared from Jurkat T cells treated with the P2 or control peptide (CP; A ) or dimethyl sulfoxide (DMSO) buffer control or RHOA inhibitor rhosin ( B ) and analyzed by Western blot analysis for total and activated RHOA using an active RHO detection kit. C and D: Quantification of Jurkat T-cell migration on denatured collagen-IV ( C ) or native collagen-IV (Nat-Coll-IV; D ) in the presence of DMSO or RHOA inhibitor rhosin. C: Data represent change in cell migration from four independent experiments with control DMSO treatment set to 100% for comparison. D: Data represent change in cell migration from three independent experiments with control DMSO treatment set to 100% for comparison. E and F: Cell lysates were prepared from Jurkat T cells treated with the P2 or CP ( E ) or DMSO or SRC non-receptor tyrosine kinase (SRC) inhibitor ( F ), and analyzed by Western blot analysis for MYO9B. G: Western blot analysis for MYO9B levels in control or MYO9B-specific siRNA-transfected Jurkat T cells. H and I: Quantification of control and MYO9B-specific knockdown Jurkat T-cell migration on denatured collagen-IV ( H ) or native collagen-IV ( I ). H: Data represent change in cell migration from four independent experiments with control siRNA treatment set to 100% for comparison. I: Data represent change in cell migration from two independent experiments with control siRNA treatment set to 100% for comparison. J: Quantification of MYO9B knockdown Jurkat T-cell migration on denatured collagen-IV in the presence of CP or P2. Data represent change in cell migration from three independent experiments with control CP treatment set to 100% for comparison. K: Example of F-actin polarization ( arrows ) in control or MYO9B-specific siRNA-transfected Jurkat T cells seeded onto denatured collagen-IV. L: Quantification of the mean percentage of F-actin polarized cells per ×400 field in control and MYO9B knockdown Jurkat T cells seeded onto denatured collagen-IV. Data represent percentage of F-actin polarized T cells from two independent experiments calculated from 10 ×400 microscopic fields per condition. Data are given as means ± SEM ( C , D , H – J , and L ). ∗ P < 0.05, ∗∗ P < 0.01. Scale bars = 20 μm ( K ).

    Journal: The American Journal of Pathology

    Article Title: Inhibiting the Secreted RGDKGE Collagen Peptide Selectively Controls CD8 + T-Cell Migration on Denatured Collagen-IV and Enhances Their Accumulation in Tumors

    doi: 10.1016/j.ajpath.2025.09.008

    Figure Lengend Snippet: RHOA and MYO9B play roles in the ability of the RGDKGE collagen peptide (P2) to modulate CD8 + T-cell migration on denatured collagen-IV (Den-Coll-IV). A and B: Whole cell lysates were prepared from Jurkat T cells treated with the P2 or control peptide (CP; A ) or dimethyl sulfoxide (DMSO) buffer control or RHOA inhibitor rhosin ( B ) and analyzed by Western blot analysis for total and activated RHOA using an active RHO detection kit. C and D: Quantification of Jurkat T-cell migration on denatured collagen-IV ( C ) or native collagen-IV (Nat-Coll-IV; D ) in the presence of DMSO or RHOA inhibitor rhosin. C: Data represent change in cell migration from four independent experiments with control DMSO treatment set to 100% for comparison. D: Data represent change in cell migration from three independent experiments with control DMSO treatment set to 100% for comparison. E and F: Cell lysates were prepared from Jurkat T cells treated with the P2 or CP ( E ) or DMSO or SRC non-receptor tyrosine kinase (SRC) inhibitor ( F ), and analyzed by Western blot analysis for MYO9B. G: Western blot analysis for MYO9B levels in control or MYO9B-specific siRNA-transfected Jurkat T cells. H and I: Quantification of control and MYO9B-specific knockdown Jurkat T-cell migration on denatured collagen-IV ( H ) or native collagen-IV ( I ). H: Data represent change in cell migration from four independent experiments with control siRNA treatment set to 100% for comparison. I: Data represent change in cell migration from two independent experiments with control siRNA treatment set to 100% for comparison. J: Quantification of MYO9B knockdown Jurkat T-cell migration on denatured collagen-IV in the presence of CP or P2. Data represent change in cell migration from three independent experiments with control CP treatment set to 100% for comparison. K: Example of F-actin polarization ( arrows ) in control or MYO9B-specific siRNA-transfected Jurkat T cells seeded onto denatured collagen-IV. L: Quantification of the mean percentage of F-actin polarized cells per ×400 field in control and MYO9B knockdown Jurkat T cells seeded onto denatured collagen-IV. Data represent percentage of F-actin polarized T cells from two independent experiments calculated from 10 ×400 microscopic fields per condition. Data are given as means ± SEM ( C , D , H – J , and L ). ∗ P < 0.05, ∗∗ P < 0.01. Scale bars = 20 μm ( K ).

    Article Snippet: To detect active RHOA, the (Rhotekin-RBD) active RHO detection kit (Cell Signaling) was used.

    Techniques: Migration, Control, Western Blot, Comparison, Transfection, Knockdown